Published in Scientific Papers. Series "Management, Economic Engineering in Agriculture and rural development", Vol. 18 ISSUE 1
Written by Widia Dwi LESTARI, Dwi Wahyudha WIRA, Gemilang Lara UTAMA
The aims of the research was to determine the occurrence of indigenous yeasts isolated from napa cabbage (Brassica rapa subsp. pekinensis) wastes potentially in produced cellulase enzyme. Indigenous yeast isolated from 1 g napa cabbage wastes with using modified Potato Dextrose Agar / PDA (Oxoid Ltd.) with the addition of 3% Yeasts Extract / YE (Kraft Foods) and 10 ppm amoxicillin. The identification of yeasts performed under microscope for size and shape then tested with Rapid Yeasts Plus System. Cellulase enzyme production was done with the addition of 2% indigenous yeasts into the substrates in the ratio of napa cabbage and water 1:15 with basal medium contains 0.05% KH2PO4 and 0.025% Mg2SO4 then incubated for 96 hours at 37ºC (modification of Gupta, et al., 2012). Every 24 hours, 1.5 ml of fermented substrates were centrifuged and the supernatant was taken as crude enzyme. The cellulose enzyme activity determined by measuring the glucose resulted using the DNS method, then tested spectroscopically using UV-Vis 9200 spectrophotometer at 540 nm. Results showed that there are 3 indigenous yeasts isolates that 2 of the isolates identified as Candida krusei and one isolate identified as Hanseniaspora guilliermondii. The cellulase enzyme activity increased in the first 24 hours then decreases until 96 hours. The best isolates activity shown by C.krusei A that resulting glucose contents of 15.9 ppm with the enzyme activity of 0.007U/ml.
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