Published in Scientific Papers. Series "Management, Economic Engineering in Agriculture and rural development", Vol. 19 ISSUE 4
Written by Mariana DUMITRU

"Candida apicola" is an osmotolerant ascomycetes yeast. Yeast in general produces secondary metabolic substances in the form of bioactive substances that can be utilized in biotechnology field, besides that yeast also has antimicrobial properties. "Candida apicola" has a cell wall that contains mannoprotein, a bioactive substance that can be used as an emulsifier in the process of making food. Beansprout extract is used as the media formula for the growth of "Candida apicola" so secure for food production. The media formula used for the growth of "Candida apicola" is derived from bean sprout extract so it is safer for food production. This study aims to determine the growth curve of "Candida apicola" on media formulated and the value of the emulsification activity of mannoprotein obtained from the extraction process. The research begin by looking at the growth curve of "Candida apicola" with an incubation time of 0 to 70th hours and observing several parameters such as optical density, pH and biomass cells. The optimal incubation time in the growth of "Candida apicola" in obtaining mannoprotein occurred at 70th hours with absorbance value of 1.5817, pH 5.69 and biomass cell 0.0271 gr/ml. The incubation time of 70th hours in 500 ml of the formulated media produced a mannoprotein of 1.5982 gr and had a 50% of emulsification activity.

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