Published in Scientific Papers. Series "Management, Economic Engineering in Agriculture and rural development", Vol. 15 ISSUE 3
Written by Eugeniu ALEXANDROV

For the isolation of the genomic deoxyribonucleic acid (DNA) from vine leaves of distant hybrids (Vitis vinifera L. x Muscadinia rotundifolia Michx.), the DNA isolation protocol based on CTAB method was used. The grouping of distant hybrids in the obtained dendrogram shows that at the DNA level there are some differences between them, differences sometimes unnoticeable at the level of ampelographic characterization. As a result, in the characterization of varieties and hybrids of vines, the ampelographic analysis needs to be supplemented by an analysis at the molecular level, based on DNA amplification techniques. As a result of distant hybrids grouping based on the size of alleles, it was found that there are two distinct main groups denoted by A and B, each having secondary branches. The hybrid F4 BC3 DRX-M4-541 1 is closely akin to the variety Chasellas dóre. Also, the two samples of Vitis vylvestris Gmel. have been found to be genetically different, being placed in different subgroups. The hybrid F4 BC3 DRX-M4-536 is genetically close to the male specimen of Vitis sylvestris Gmel. The distant hybrid DRX-M4-660, which proved to have larger differences at the molecular level, isn’t grouped in a cluster with any other hybrid.

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