Published in Scientific Papers. Series "Management, Economic Engineering in Agriculture and rural development", Vol. 21 ISSUE 4
Written by Elena BONCIU, Recep LIMAN, İbrahim Hakki CIGERCI

The regeneration capacity of the cells of an explant depends on several factors: the nature and origin of the explants, their type, structure and degree of juvenility, cell maturity and physiological state, endogenous phytohormone content, composition of culture medium and culture conditions, etc. Among them, the culture medium is an essential factor for the success of in vitro culture. In its composition, in addition to macro and microelements, vitamins, sucrose, etc., can be added some natural nutritional extracts, such as deproteinated coconut milk or various vegetable and fruit juices, to improve the complexity of the culture medium. This paper presents the results obtained in the laboratory from the in vitro culture of okra (Hibiscus esculentus), on modified Murashige-Skoog culture medium (MS) by adding natural orange juice in three different concentrations: 5, 10 and 20%. It was observed that at the 10% concentration of orange juice were registered the highest values, both in terms of germination, explant growth and mitotic activity. The lowest values from this point of view were obtained at a concentration of 20%. These results suggest the nutritional potential of orange juice added to the MS culture medium to increase the growth rate and in vitro survival of okra via cells competence improvement.

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